6GQG

Structure of GFPmut2 crystallized at pH 8.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	51.166, 62.375, 69.767
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.500 - 1.792
R-factor	0.1846
Rwork	0.183
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q4a
RMSD bond length	0.006
RMSD bond angle	1.084
Data reduction software	XDS
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.500	46.500	1.890
High resolution limit [Å]	1.792	5.670	1.792
Rmerge		0.026	0.541
Rmeas	0.077	0.028	0.599
Rpim	0.030	0.011	0.249
Number of reflections	21218	777	2805
<I/σ(I)>	18	22.8	1.4
Completeness [%]	98.3	99.6	91.3
Redundancy	6.3	6.4	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	277	0.2 M CaCl2, 19% PEG 4000