Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.880, 82.010, 91.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.100 - 1.900 |
| R-factor | 0.2077 |
| Rwork | 0.206 |
| R-free | 0.24040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dqi |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.501 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.100 | 1.950 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.054 | 0.383 |
| Rmeas | 0.062 | 0.453 |
| Rpim | 0.030 | 0.237 |
| Number of reflections | 31984 | 2864 |
| <I/σ(I)> | 14.1 | 2.6 |
| Completeness [%] | 98.6 | 91.6 |
| Redundancy | 4.1 | 3.1 |
| CC(1/2) | 0.998 | 0.858 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 25% (w/v) PEG 2000 MME |
