6GQ4
Neisseria gonorrhoeae Adhesin Complex Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-03 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.07227 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 49.092, 30.936, 67.572 |
| Unit cell angles | 90.00, 103.47, 90.00 |
Refinement procedure
| Resolution | 32.880 - 1.650 |
| R-factor | 0.12818 |
| Rwork | 0.125 |
| R-free | 0.17944 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.783 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.880 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.170 | |
| Rmeas | 0.182 | |
| Rpim | 0.066 | |
| Number of reflections | 12098 | 593 |
| <I/σ(I)> | 14.4 | 6.4 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 7.6 | 7.7 |
| CC(1/2) | 0.987 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Potassium Thionate, 30% W/V PEG2000 MME, pH 7.5 |
