6GQ0

Crystal structure of GanP, a glucose-galactose binding protein from Geobacillus stearothermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 31 2 1
Unit cell lengths	67.719, 67.719, 152.173
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.365 - 1.940
R-factor	0.1985
Rwork	0.197
R-free	0.22790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3c6q
RMSD bond length	0.008
RMSD bond angle	0.932
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.360	1.990
High resolution limit [Å]	1.940	1.940
Rmerge	0.128	0.564
Number of reflections	30682	2021
<I/σ(I)>	8	2.2
Completeness [%]	99.8	99.6
Redundancy	6.5	5.9
CC(1/2)	0.991	0.939

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1 M HEPES (pH 7.5 buffer), and 20% w/v PEG 10K.