6GPZ
Cell division regulator GpsB in complex with peptide fragment of L. monocytogenes Penicillin Binding Protein PBPA1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 26.577, 31.415, 81.032 |
Unit cell angles | 90.00, 92.41, 90.00 |
Refinement procedure
Resolution | 29.288 - 1.600 |
R-factor | 0.1747 |
Rwork | 0.173 |
R-free | 0.20400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6gp7 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.372 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.420 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rpim | 0.044 | 0.386 |
Number of reflections | 17944 | 902 |
<I/σ(I)> | 8.4 | 1.8 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 3.5 | 3.3 |
CC(1/2) | 0.997 | 0.743 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M imidazole pH 8, 0.2M zinc acetate, 20% PEG 3000 |