6GPN
Crystal Structure of the CsiD Glutarate Hydroxylase in complex with N-Oxalylglycine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.000 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 121.380, 121.380, 136.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.914 - 2.200 |
R-factor | 0.1873 |
Rwork | 0.187 |
R-free | 0.21140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6gpe |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.310 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 91254 | |
<I/σ(I)> | 17.7 | |
Completeness [%] | 100.0 | |
Redundancy | 26.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 3.5 M Sodium formate, 0.1 M BIS-TRIS propane pH 7.0 |