6GPM
Crystal structure of domain 2 from TmArgBP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-02 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.619, 49.730, 27.782 |
| Unit cell angles | 90.00, 100.19, 90.00 |
Refinement procedure
| Resolution | 14.180 - 1.730 |
| R-factor | 0.13137 |
| Rwork | 0.129 |
| R-free | 0.16847 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4prs |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.674 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.770 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.040 | |
| Number of reflections | 6898 | |
| <I/σ(I)> | 30.2 | |
| Completeness [%] | 95.1 | |
| Redundancy | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Crystals of the D2 domain were obtained using a protein concentration of 14 mg/ml in a solution containing 0.1M Bis-Tris (pH 6.5) and 28% (w/v) polyethylene glycol 2,000. |
