6GPB

REFINED CRYSTAL STRUCTURE OF THE PHOSPHORYLASE-HEPTULOSE 2-PHOSPHATE-OLIGOSACCHARIDE-AMP COMPLEX

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	128.500, 128.500, 116.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.860
Rwork	0.201
RMSD bond length	0.022
RMSD bond angle	3.400
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.860 *
Rmerge	0.063 *
Total number of observations	88108 *
Number of reflections	18715 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	6.7 *	16 *	took Mclaughlin et al., 1984 from original paper *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	phosphorylase b	25 (mg/ml)
2	1	1	IMP	2 (mM)
3	1	1	EDTA	0.1 (mM)