6GOZ

Structure of mEos4b in the green long-lived dark state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97903
Spacegroup name	P 21 21 21
Unit cell lengths	38.620, 57.660, 102.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.285 - 2.406
R-factor	0.208
Rwork	0.202
R-free	0.26420
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS (Version June 1, 2017)
Data scaling software	XSCALE (Version June 1, 2017)
Phasing software	PHENIX (1.15)
Refinement software	PHENIX (1.15)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.285	38.285	2.470
High resolution limit [Å]	2.406	10.760	2.410
Rmerge	0.131	0.035	0.551
Rmeas	0.151	0.041	0.639
Number of reflections	8804	107	634
<I/σ(I)>	9.53	28.12	2.58
Completeness [%]	94.1	81.7	95.3
Redundancy	3.451	3.393	3.521
CC(1/2)	0.994	0.997	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	100 mM HEPES, 30 % PEG 1000