6GOW
Crystal structure of the flagellin-FliS complex from Bacillus subtilis crystallized in spacegroup P22121
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 50.860, 89.956, 116.079 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.770 - 2.100 |
R-factor | 0.1769 |
Rwork | 0.175 |
R-free | 0.20390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5maw |
RMSD bond length | 0.003 |
RMSD bond angle | 0.513 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.770 | 2.175 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmeas | 0.083 | 0.155 |
Number of reflections | 31628 | 3022 |
<I/σ(I)> | 13.65 | 7.17 |
Completeness [%] | 99.3 | 97.61 |
Redundancy | 4.2 | 4 |
CC(1/2) | 0.995 | 0.974 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 M Na-Cacodylate pH 6.5, 5% PEG 8000, 40 % MPD |