6GOE
KRAS full length G12V GPPNHP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-16 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.928 |
| Spacegroup name | H 3 |
| Unit cell lengths | 79.880, 79.880, 78.790 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.940 - 1.600 |
| R-factor | 0.18373 |
| Rwork | 0.182 |
| R-free | 0.22007 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dst |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.071 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.940 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.088 | 1.070 |
| Rmeas | 0.098 | |
| Rpim | 0.043 | 0.526 |
| Number of reflections | 24651 | 1820 |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 5.1 | 5.1 |
| CC(1/2) | 0.996 | 0.528 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.1 M TrisCl, 0.2 M NaOAc and 30-35 % PEG 4000 |
