6GO4

TdT chimera (Loop1 of pol mu) - binary complex with ddCTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	46.970, 85.620, 114.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.710 - 1.960
R-factor	0.203
Rwork	0.201
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jms
RMSD bond length	0.010
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.780	2.070
High resolution limit [Å]	1.960	1.960
Rmerge	0.101	1.666
Number of reflections	34181	5333
<I/σ(I)>	11.02
Completeness [%]	99.7	98.5
Redundancy	6.5
CC(1/2)	0.998	0.498

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	20-24% PEG 6000, 400-800 mM lithium chloride, 100 mM MES