6GN6
Alpha-L-fucosidase isoenzyme 1 from Paenibacillus thiaminolyticus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-11 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9201 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 107.772, 148.758, 194.895 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.930 - 2.200 |
R-factor | 0.169 |
Rwork | 0.167 |
R-free | 0.21700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wvs |
RMSD bond length | 0.016 |
RMSD bond angle | 1.825 |
Data reduction software | XDS (November 3, 2014) |
Data scaling software | XDS (November 3, 2014) |
Phasing software | MOLREP (11.2.08) |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.930 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.099 | 0.709 |
Number of reflections | 157780 | |
<I/σ(I)> | 12.3 | 2.1 |
Completeness [%] | 99.4 | 99.2 |
Redundancy | 5 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291.15 | 25% (w/v) PEG 3350, 0.2 M Ammonium acetate, 0.1 M BIS-TRIS buffer, Additive: 50 mM Maltose |