6GMZ
Staphylopine dehydrogenase in complex with Histidine tag and citrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-31 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 186.440, 48.790, 60.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.200 - 2.220 |
R-factor | 0.18403 |
Rwork | 0.181 |
R-free | 0.24889 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.825 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | CRANK |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 52360 | |
<I/σ(I)> | 13.7 | |
Completeness [%] | 98.6 | |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M sodium citrate tribasic dihydrate, 20% PEG 3350 |