6GMO
Plant glutamate cysteine ligase (GCL) in complex with non-reducing GSH (GSM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-01 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.975 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.770, 109.860, 84.750 |
| Unit cell angles | 90.00, 98.72, 90.00 |
Refinement procedure
| Resolution | 19.716 - 1.750 |
| R-factor | 0.1651 |
| Rwork | 0.163 |
| R-free | 0.19750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gwd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.397 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.890 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 105763 | |
| <I/σ(I)> | 14.09 | |
| Completeness [%] | 98.9 | |
| Redundancy | 3.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M NaAc, 20 % (w/v) PEG 3,350 |
