6GLF
Crystal structure of hMTH1 F27A in the presence of acetate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-21 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 35.975, 60.382, 65.581 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.421 - 2.000 |
| R-factor | 0.1897 |
| Rwork | 0.183 |
| R-free | 0.24730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.799 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.038 | 0.106 |
| Number of reflections | 17566 | 2733 |
| <I/σ(I)> | 18.08 | 8.13 |
| Completeness [%] | 94.2 | 90.4 |
| Redundancy | 1.84 | 1.67 |
| CC(1/2) | 0.999 | 0.984 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 23-27% PEG3350, 0.2 M LiSO4, 0.1 M sodium acetate pH 4.5 |
