6GL2
Structure of ZgEngAGH5_4 wild type at 1.2 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.984 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.490, 48.460, 59.260 |
| Unit cell angles | 90.00, 104.35, 90.00 |
Refinement procedure
| Resolution | 57.410 - 1.960 |
| R-factor | 0.17348 |
| Rwork | 0.172 |
| R-free | 0.20050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ndz |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.134 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.410 | 1.240 |
| High resolution limit [Å] | 1.180 | 1.180 |
| Rmerge | 0.074 | 0.740 |
| Rmeas | 0.160 | 0.740 |
| Number of reflections | 99084 | 14405 |
| <I/σ(I)> | 10.4 | 1.8 |
| Completeness [%] | 98.0 | 98 |
| Redundancy | 6.3 | 6.3 |
| CC(1/2) | 0.990 | 0.430 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 296 | 14% PEG 6000, 200 mM CaCl2, 100 mM M sodium acetate buffer at pH 5.0 |
