6GL1
HLA-E*01:03 in complex with the HIV epitope, RL9HIV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 244.820, 48.220, 153.020 |
| Unit cell angles | 90.00, 117.38, 90.00 |
Refinement procedure
| Resolution | 135.878 - 2.623 |
| R-factor | 0.2259 |
| Rwork | 0.223 |
| R-free | 0.27280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gh1 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.616 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 135.878 | 2.660 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 2.345 | |
| Number of reflections | 48641 | 2439 |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.447 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293.5 | 2.4 M Ammonium Sulphate, 0.1 M MES, pH 6, 0.05% dichloromethane |
