6GKS

X-ray structure determined from recombinant Charcot-Leyden crystal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 65 2 2
Unit cell lengths	48.900, 48.900, 258.629
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.105 - 1.380
R-factor	0.1686
Rwork	0.168
R-free	0.17940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lcl
RMSD bond length	0.005
RMSD bond angle	0.909
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.420
High resolution limit [Å]	1.340	1.340
Rmeas	0.076	0.720
Number of reflections	39313	4579
<I/σ(I)>	25.64	5.8
Completeness [%]	92.4	68.3
Redundancy	17.9	14.6
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7.4	298	Upon removal of the N-terminal His-tag by TEV protease, at a protease:target protein ratio of 1:100 (w/w), recombinant galectin-10 autocrystallized in PBS buffer.