6GKJ

Translation initiation factor 4E in complex with trinucleotide mRNA 5' cap (m7GpppApG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-01
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	0.9184
Spacegroup name	P 1
Unit cell lengths	38.190, 38.190, 147.020
Unit cell angles	84.18, 88.55, 76.96

Refinement procedure

Resolution	24.074 - 2.068
R-factor	0.2014
Rwork	0.200
R-free	0.24860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5m7x
RMSD bond length	0.007
RMSD bond angle	0.923
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.760	2.120
High resolution limit [Å]	2.068	2.068
Rmerge	0.194	0.585
Rmeas	0.214	0.715
Number of reflections	46288	3273
<I/σ(I)>	7.08	1.65
Completeness [%]	94.1	88.7
Redundancy	4.343	2.966
CC(1/2)	0.987	0.637

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	15% PEG 10000, 0.1M TrisHCl pH 8.0, 0.2M CH3COONH4