6GKI
Structure of E coli MlaC in Variously Loaded States
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-05 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97951 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 105.290, 105.290, 96.410 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 52.640 - 2.230 |
R-factor | 0.1991 |
Rwork | 0.197 |
R-free | 0.23640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5uwa |
RMSD bond length | 0.014 |
RMSD bond angle | 1.571 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0216) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 52.640 | 52.640 | 2.290 |
High resolution limit [Å] | 2.230 | 9.970 | 2.230 |
Rmerge | 0.113 | 0.038 | 2.492 |
Rmeas | 0.117 | 0.039 | 2.603 |
Rpim | 0.027 | 0.009 | 0.722 |
Total number of observations | 559345 | ||
Number of reflections | 30115 | 398 | 1978 |
<I/σ(I)> | 17.9 | ||
Completeness [%] | 98.7 | 99.5 | 89 |
Redundancy | 18.6 | 16.2 | 12.1 |
CC(1/2) | 0.999 | 0.999 | 0.490 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | seed grown in (0.1M Na Cacodylate pH 6.5, 2M ammonium sulphate, 0.2M NaCl) seed transferred to drop of (0.09M NaF/Br/I, 0.1M Tris/bicine pH 8.5, 20% glycerol, 10% PEG 4000) |