6GJZ
CryoEM structure of the MDA5-dsRNA filament in complex with AMPPNP
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
Method | HELICAL |
Resolution (Å) | 4.06 |
Number of Particle | 207000 |
Software | RELION,PHENIX,EPU,Gctf,Coot |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |