6GJ4
Tubulin-6j complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-14 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00001 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.970, 157.810, 181.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.022 - 2.400 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.24060 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4i4t |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.628 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.022 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.146 | 2.499 |
| Rmeas | 0.158 | 2.706 |
| Rpim | 0.064 | 1.105 |
| Number of reflections | 117973 | 8642 |
| <I/σ(I)> | 8.7 | 0.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | 6.7 |
| CC(1/2) | 0.996 | 0.346 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.7 | 293 | 10% PEG 4K, 12.5% GLYCEROL, 30 MM MAGNESIUM CHLORIDE, 30 MM CALCIUM CHLORIDE, 0.1M MES / 0.1M IMIDAZOLE |
