6GIO
Structure of Amino Acid Amide Racemase from Ochrobactrum anthropi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2015-07-25 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.600, 134.980, 88.770 |
| Unit cell angles | 90.00, 90.25, 90.00 |
Refinement procedure
| Resolution | 64.600 - 1.870 |
| R-factor | 0.18715 |
| Rwork | 0.185 |
| R-free | 0.23047 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5m46 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.519 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.600 | 1.920 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.110 | 0.920 |
| Rpim | 0.090 | 0.770 |
| Number of reflections | 123230 | 9034 |
| <I/σ(I)> | 7.6 | 1.4 |
| Completeness [%] | 98.5 | 97.9 |
| Redundancy | 4.2 | 4.2 |
| CC(1/2) | 1.000 | 0.550 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1 M HEPES, pH 7.0 2ith 0.2 M MgCl2 and 25% (w/v) PEG 3350 |
