6GI4
Structure of Lytic Transglycosylase MltE mutant S75A from E.coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979210 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 56.636, 56.636, 110.434 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.050 - 1.350 |
| R-factor | 0.18583 |
| Rwork | 0.184 |
| R-free | 0.21554 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y8p |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.711 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.050 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rpim | 0.018 | |
| Number of reflections | 40280 | |
| <I/σ(I)> | 18.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 20% Ethanol and 0.1 M TRIS HCl (pH 8.5) |
