6GHR
cyanobacterial GAPDH with full-length CP12
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97951 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 133.438, 146.964, 81.428 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 71.226 - 2.249 |
R-factor | 0.1918 |
Rwork | 0.190 |
R-free | 0.22660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4boy |
RMSD bond length | 0.002 |
RMSD bond angle | 0.565 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.230 | 2.329 |
High resolution limit [Å] | 2.249 | 2.249 |
Rmerge | 0.099 | 0.463 |
Rmeas | 0.116 | 0.565 |
Number of reflections | 76310 | 7510 |
<I/σ(I)> | 7.68 | 1.82 |
Completeness [%] | 99.3 | 98.79 |
Redundancy | 3.5 | 2.9 |
CC(1/2) | 0.993 | 0.709 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 290 | 4% Tacsimate, pH 7 12% (w/v) PEG3350 |