6GHB
Crystal structure of Spx in complex with YjbH (oxidized)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-08 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.596, 88.704, 117.467 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.429 - 3.104 |
R-factor | 0.226 |
Rwork | 0.222 |
R-free | 0.26590 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.107 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: 000)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.429 | 3.200 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 16640 | 1613 |
<I/σ(I)> | 10.97 | 1.68 |
Completeness [%] | 99.2 | 98.59 |
Redundancy | 4.1 | 4.1 |
CC(1/2) | 0.994 | 0.609 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Na-HEPES pH 7.5, MgCl2 and PEG400 |