6GH3
Paenibacillus sp. YM1 laminaribiose phosphorylase with alpha-man-1-phosphate bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 146.570, 146.570, 222.227 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.290 - 1.820 |
| R-factor | 0.1952 |
| Rwork | 0.194 |
| R-free | 0.21850 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 6ggy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 73.290 | 73.290 | 1.850 |
| High resolution limit [Å] | 1.820 | 9.970 | 1.820 |
| Rmerge | 0.119 | 0.031 | 1.992 |
| Rmeas | 0.126 | 0.033 | 2.108 |
| Rpim | 0.041 | 0.011 | 0.680 |
| Total number of observations | 1928822 | ||
| Number of reflections | 215182 | 1525 | 10580 |
| <I/σ(I)> | 12.7 | ||
| Completeness [%] | 100.0 | 99.6 | 100 |
| Redundancy | 9 | 7.6 | 9.2 |
| CC(1/2) | 0.999 | 0.999 | 0.407 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Null |
