6GGR

Crystal structure of Salmonella zinc metalloprotease effector GtgA in complex with p65

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9159
Spacegroup name	P 21 21 21
Unit cell lengths	39.392, 85.833, 111.869
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.930 - 2.097
R-factor	0.2084
Rwork	0.206
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ram
RMSD bond length	0.006
RMSD bond angle	0.645
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.930	2.172
High resolution limit [Å]	2.097	2.097
Rmerge	0.189
Rmeas	0.193
Rpim	0.035
Number of reflections	22948
<I/σ(I)>	13.9
Completeness [%]	99.0
Redundancy	31.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Crystallyzation conditions: 0.1 M Tris pH 8.3, 0.5 M LiCl and 32.5% (w/v) PEG 6000