6GGP
Structure of the ligand-free form of truncated ArgBP (residues 20-233) from T. maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9464 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.707, 71.063, 88.386 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.030 |
| R-factor | 0.138 |
| Rwork | 0.137 |
| R-free | 0.17100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4prs |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.183 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.050 |
| High resolution limit [Å] | 1.030 | 1.030 |
| Number of reflections | 104443 | |
| <I/σ(I)> | 41.3 | |
| Completeness [%] | 95.9 | |
| Redundancy | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Crystals grew in 16-20 mg/mL protein solution and in 30 % w/v PEG 8,000, 0.1 M Sodium cacodylate trihydrate (pH 6.5) and 0.2 M Sodium Acetate trihydrate. |
