6GGO
Crystal structure of Salmonella zinc metalloprotease effector GtgA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 95.783, 40.678, 112.161 |
| Unit cell angles | 90.00, 94.00, 90.00 |
Refinement procedure
| Resolution | 75.300 - 2.600 |
| R-factor | 0.2192 |
| Rwork | 0.217 |
| R-free | 0.25530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.506 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.300 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.102 | |
| Rmeas | 0.120 | |
| Rpim | 0.067 | |
| Number of reflections | 13578 | |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 98.4 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | GtgA, at a concentration of 24 mg/ml, was crystallyzed in 0.1 M Tris pH 8.5, 25% (v/v) isopropanol and 20% (w/v) PEG 3350 |
