6GFO
cyanobacterial GAPDH with full-length CP12
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-12 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 136.446, 141.049, 81.592 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 70.627 - 2.100 |
R-factor | 0.1731 |
Rwork | 0.171 |
R-free | 0.21850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4boy |
RMSD bond length | 0.007 |
RMSD bond angle | 0.905 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.630 | 2.175 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.139 | 0.496 |
Rmeas | 0.151 | 0.542 |
Rpim | 0.060 | |
Number of reflections | 92479 | 9129 |
<I/σ(I)> | 6.9 | 2.45 |
Completeness [%] | 99.9 | 99.91 |
Redundancy | 6.3 | 6.2 |
CC(1/2) | 0.991 | 0.905 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 290 | 1% Tryptone, 0.001 M Azide, 0.005 M Azide, 12 % PEG3350 |