6GFN

METTL16 MTase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-02
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.8731
Spacegroup name	I 41 2 2
Unit cell lengths	92.360, 92.360, 180.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	82.230 - 2.860
R-factor	0.183
Rwork	0.181
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6gfk
RMSD bond length	0.010
RMSD bond angle	1.110
Data reduction software	XDS (Nov 11, 2017 (BUILT 20171111))
Data scaling software	Aimless (0.6.2)
Phasing software	PHASER (2.7.17)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.240	3.200
High resolution limit [Å]	2.850	2.850
Rmerge	0.177	1.320
Rmeas	0.192	1.420
Rpim	0.073	0.500
Number of reflections	5724	286
<I/σ(I)>	8	1.8
Completeness [%]	93.1	78.7
Redundancy	6.9	7.8
CC(1/2)	0.996	0.590

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M di-sodium tartrate and 20% (w/v) PEG 3350