6GFC

Structure of the BTB/POZ domain of human 90K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97630
Spacegroup name	I 41 2 2
Unit cell lengths	181.740, 181.740, 173.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.300
R-factor	0.2678
Rwork	0.267
R-free	0.28660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6gfb
RMSD bond length	0.007
RMSD bond angle	0.930
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.390
High resolution limit [Å]	3.300	3.300
Rmeas	0.140	0.824
Number of reflections	22154
<I/σ(I)>	21.7	1.05
Completeness [%]	100.0	99.9
Redundancy	56.9
CC(1/2)	1.000	0.394

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	29% PEG 4000 0.2M MgCl2 0.1M Tris (pH 8.5) 10% sucrose