6GF6
Molecular basis of egg coat filament cross-linking: high-resolution structure of the partially deglycosylated ZP1 ZP-N1 domain homodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.7710 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 75.980, 75.980, 101.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.966 - 2.300 |
| R-factor | 0.1958 |
| Rwork | 0.192 |
| R-free | 0.22920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Partially refined model derived from SAD phasing of a gold derivative |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.738 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_3409: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.990 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.116 | 1.498 |
| Rpim | 0.026 | 0.495 |
| Number of reflections | 13550 | 1422 |
| <I/σ(I)> | 22.2 | 1.4 |
| Completeness [%] | 98.0 | 84.6 |
| Redundancy | 19.7 | 8.3 |
| CC(1/2) | 1.000 | 0.550 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.6 M LiCl, 0.1 M tri-sodium citrate pH 5.5 |
