6GF1
The structure of the ubiquitin-like modifier FAT10 reveals a novel targeting mechanism for degradation by the 26S proteasome
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.999 |
Spacegroup name | H 3 |
Unit cell lengths | 116.172, 116.172, 84.720 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.254 - 1.925 |
R-factor | 0.211 |
Rwork | 0.210 |
R-free | 0.23310 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.810 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.254 | 1.994 |
High resolution limit [Å] | 1.920 | 1.925 |
Number of reflections | 64119 | |
<I/σ(I)> | 21.38 | |
Completeness [%] | 99.3 | |
Redundancy | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293.15 | 3.2 M Ammonium sulfate, 0.1 M Citric acid |