6GF0
Lysozyme structure determined from SFX data using a Sheet-on-Sheet chipless chip
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL3 |
| Synchrotron site | SACLA |
| Beamline | BL3 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2017-12-18 |
| Detector | MPCCD |
| Wavelength(s) | 1.770 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.100, 79.100, 39.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.550 - 2.070 |
| R-factor | 0.20034 |
| Rwork | 0.199 |
| R-free | 0.23483 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4n5r |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.962 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.200 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Number of reflections | 7417 | |
| <I/σ(I)> | 10.3 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 283 | 42 |
| CC(1/2) | 0.986 | 0.773 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293 | 20 % NaCl, 6 % PEG 6000, 0.1 M sodium acetate pH 3.0 |
