6GEU
Crystal structure of the C230A mutant of human IBA57
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-12 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.973 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.360, 43.060, 58.250 |
| Unit cell angles | 76.27, 76.17, 70.94 |
Refinement procedure
| Resolution | 35.690 - 1.550 |
| R-factor | 0.16184 |
| Rwork | 0.160 |
| R-free | 0.20332 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ESR |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.902 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.690 | 1.590 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.052 | 0.580 |
| Number of reflections | 49014 | 7712 |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 89.8 | 87.7 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M MES, 20% PEG 3350 |
