6GEL
The structure of TWITCH-2B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.075, 156.773, 169.284 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.930 - 2.510 |
| R-factor | 0.1975 |
| Rwork | 0.195 |
| R-free | 0.23960 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.005 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.930 | 2.610 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Rmeas | 0.033 | 0.241 |
| Number of reflections | 50452 | |
| <I/σ(I)> | 24.16 | 3.32 |
| Completeness [%] | 98.7 | |
| Redundancy | 6.62 | 6.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 0.2 M sodium formiate, 5 mM calcium chloride, 18 % PEG 3350 |
