6GEH
Structure and reactivity of a siderophore-interacting protein from the marine bacterium Shewanella reveals unanticipated functional versatility.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.098, 77.753, 44.917 |
| Unit cell angles | 90.00, 109.64, 90.00 |
Refinement procedure
| Resolution | 28.620 - 1.150 |
| R-factor | 0.1463 |
| Rwork | 0.145 |
| R-free | 0.17350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yhb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.384 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.620 | 1.190 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Number of reflections | 86403 | |
| <I/σ(I)> | 18.4 | |
| Completeness [%] | 99.0 | |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | PEG 3350, Magnesium formate dihydrate |
