6GE9
Structure of Mycobacterium tuberculosis GlmU bound to Glc-1P and Ac-CoA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97 |
Spacegroup name | H 3 2 |
Unit cell lengths | 109.753, 109.753, 364.894 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.126 - 2.260 |
R-factor | 0.245 |
Rwork | 0.243 |
R-free | 0.27650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4g87 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.567 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.710 | 2.340 |
High resolution limit [Å] | 2.260 | 2.260 |
Rmerge | 0.070 | 1.070 |
Rpim | 0.460 | |
Number of reflections | 40178 | 3861 |
<I/σ(I)> | 9.6 | |
Completeness [%] | 98.3 | 96.6 |
Redundancy | 6.5 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.05 M ADA pH 6.8, 5.7% PEG 550 MME, 32.1% PEG 200 |