6GE4

TEAD4 (216-434);E263A COMPLEXED WITH YAP PEPTIDE (60-100) AND MYRISTOATE (COVALENTLY BOUND) AT 1.97A (P41212 CRYSTAL FORM); MYRISTOYLATION WAS DONE BY ADDING MYR-COA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99993
Spacegroup name	P 41 21 2
Unit cell lengths	59.033, 59.033, 159.129
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.730 - 1.970
R-factor	0.2244
Rwork	0.224
R-free	0.24110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ge3
RMSD bond length	0.006
RMSD bond angle	0.958
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.730	2.020
High resolution limit [Å]	1.970	1.970
Rmerge	0.088	1.007
Rmeas	0.092	1.047
Number of reflections	20703
<I/σ(I)>	22.8	3.9
Completeness [%]	99.8	100
Redundancy	12.7	13.3
CC(1/2)	0.999	0.937

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% PEG 3350, 0.2M MgNitrate