6GE4
TEAD4 (216-434);E263A COMPLEXED WITH YAP PEPTIDE (60-100) AND MYRISTOATE (COVALENTLY BOUND) AT 1.97A (P41212 CRYSTAL FORM); MYRISTOYLATION WAS DONE BY ADDING MYR-COA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99993 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 59.033, 59.033, 159.129 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.730 - 1.970 |
| R-factor | 0.2244 |
| Rwork | 0.224 |
| R-free | 0.24110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ge3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.958 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.730 | 2.020 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.088 | 1.007 |
| Rmeas | 0.092 | 1.047 |
| Number of reflections | 20703 | |
| <I/σ(I)> | 22.8 | 3.9 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 12.7 | 13.3 |
| CC(1/2) | 0.999 | 0.937 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% PEG 3350, 0.2M MgNitrate |
