6GDS
Holliday Junctions formed from Telomeric DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-27 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.92016 |
Spacegroup name | P 1 |
Unit cell lengths | 23.334, 33.183, 41.303 |
Unit cell angles | 99.27, 90.00, 110.59 |
Refinement procedure
Resolution | 15.740 - 2.950 |
R-factor | 0.2441 |
Rwork | 0.242 |
R-free | 0.28630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hs1 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.457 |
Data reduction software | CrysalisPro (1.171.37.34) |
Data scaling software | Aimless (0.6.3) |
Phasing software | PHASER |
Refinement software | REFMAC (refmac_5.8.0222) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 15.740 | 15.360 | 2.950 |
High resolution limit [Å] | 2.800 | 8.860 | 2.800 |
Rmerge | 0.114 | 0.094 | 0.336 |
Rmeas | 0.161 | 0.133 | 0.475 |
Rpim | 0.114 | 0.094 | 0.336 |
Total number of observations | 101 | 726 | |
Number of reflections | 2615 | 58 | 408 |
<I/σ(I)> | 5.6 | 11.3 | 1.5 |
Completeness [%] | 92.9 | 63 | 96.5 |
Redundancy | 1.8 | 1.7 | 1.8 |
CC(1/2) | 0.947 | 0.943 | 0.952 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 285 | 25 mM Potassium Chloride, 10 mM Potassium Cacodylate, 50mM Magnesium Chloride, 20mM Calcium Chloride, 35% v/v (+/-)-2-Methyl-2,4-Pentanediol. |