6GDH
Holliday Junctions formed from Telomeric DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | Agilent SuperNova |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2014-11-12 |
Detector | AGILENT TITAN CCD |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 23.733, 33.161, 83.467 |
Unit cell angles | 99.87, 90.07, 110.94 |
Refinement procedure
Resolution | 22.510 - 2.850 |
R-factor | 0.2298 |
Rwork | 0.228 |
R-free | 0.27290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hs1 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.552 |
Data reduction software | CrysalisPro (1.171.36.32) |
Data scaling software | Aimless (0.6.3) |
Phasing software | PHASER |
Refinement software | REFMAC (refmac_5.8.0222) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 22.510 | 22.510 | 2.950 |
High resolution limit [Å] | 2.800 | 8.860 | 2.800 |
Rmerge | 0.059 | 0.026 | 0.447 |
Rmeas | 0.070 | 0.031 | 0.555 |
Rpim | 0.037 | 0.016 | 0.324 |
Total number of observations | 19984 | ||
Number of reflections | 5625 | 169 | 813 |
<I/σ(I)> | 11.4 | ||
Completeness [%] | 97.8 | 88.9 | 93.6 |
Redundancy | 3.6 | 3.9 | 2.6 |
CC(1/2) | 0.998 | 0.999 | 0.915 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 285 | 25 mM potassium chloride, 10 mM potassium cacodylate 50mM for magnesium chloride, 20mM for calcium chloride, MPD 32% to 38% |