6GD7
Cytochrome c in complex with Sulfonato-calix[8]arene, H3 form with PEG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-09 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980 |
| Spacegroup name | H 3 |
| Unit cell lengths | 81.420, 81.420, 82.364 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.710 - 1.550 |
| R-factor | 0.151 |
| Rwork | 0.149 |
| R-free | 0.18350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5LYC chain A |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.437 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.710 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmeas | 0.056 | 0.516 |
| Rpim | 0.026 | 0.256 |
| Number of reflections | 29520 | 1434 |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.4 | 3.9 |
| CC(1/2) | 1.000 | 0.490 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 5 % PEG 3350 (2x protein solution : 1x crystallisation condition) |
