6GCT
cryo-EM structure of the human neutral amino acid transporter ASCT2
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
EM Data Collection
| Microscope | FEI TALOS ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Quantum LS |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.85 |
| Software | RELION,EPU,CTFFIND,Coot,PHENIX |
| Symmetry | C3 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
