6GCM
Escherichia coli DPS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.972422 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 150.150, 89.420, 150.650 |
| Unit cell angles | 90.00, 90.24, 90.00 |
Refinement procedure
| Resolution | 100.020 - 2.450 |
| R-factor | 0.20547 |
| Rwork | 0.203 |
| R-free | 0.25805 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dps |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.583 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 150.650 | 2.514 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Number of reflections | 138619 | |
| <I/σ(I)> | 8.11 | |
| Completeness [%] | 99.2 | |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION, RECRYSTALLIZATION | 291 | PEG 8000 |
