6GCF
DNA binding domain of restriction endonuclease McrBC in complex with N4-methylcytosine DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-26 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.280, 69.675, 145.054 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.810 - 1.550 |
R-factor | 0.1389 |
Rwork | 0.134 |
R-free | 0.17840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ssc |
RMSD bond length | 0.018 |
RMSD bond angle | 1.457 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.810 | 1.605 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.040 | 0.402 |
Number of reflections | 108317 | 10618 |
<I/σ(I)> | 28.36 | 5.38 |
Completeness [%] | 99.9 | 99.16 |
Redundancy | 6.5 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M LiCl, 17% PEG4000 |