6GBV

A fast recovering full-length LOV protein (DsLOV) from the marine phototrophic bacterium Dinoroseobacter shibae (Dark state) - M49T mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-17
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	C 1 2 1
Unit cell lengths	90.076, 31.086, 49.411
Unit cell angles	90.00, 112.88, 90.00

Refinement procedure

Resolution	26.200 - 1.630
R-factor	0.1505
Rwork	0.148
R-free	0.19760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kuk
RMSD bond length	0.005
RMSD bond angle	0.749
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.520	1.660
High resolution limit [Å]	1.630	1.630
Rmerge	0.064	0.708
Rmeas	0.072	0.794
Number of reflections	15724	760
<I/σ(I)>	13.2	2.1
Completeness [%]	98.4	97.1
Redundancy	4.7	4.8
CC(1/2)	0.999	0.699

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292.15	0.1 M HEPES sodium, 0.8 M Sodium phosphate monobasic monohydrate, 0.8 M Potassium phosphate monobasic