6GBR

Crystal Structure of the oligomerization domain of VP35 from Reston virus, mercury derivative

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-20
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.00904
Spacegroup name	P 21 21 21
Unit cell lengths	35.933, 91.901, 104.193
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.579 - 3.150
R-factor	0.2125
Rwork	0.207
R-free	0.31130
Structure solution method	SAD
RMSD bond length	0.023
RMSD bond angle	1.896
Data reduction software	XDS (VERSION Nov 1, 2016)
Data scaling software	Aimless (0.5.28)
Phasing software	SHELXDE (2013/2)
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.950	45.950	3.350
High resolution limit [Å]	3.140	8.870	3.140
Rmerge	0.147	0.072	1.111
Rmeas	0.149	0.073	1.128
Rpim	0.022	0.011	0.181
Total number of observations	305959
Number of reflections	6505	340	1132
<I/σ(I)>	21.4
Completeness [%]	99.5	99.6	97.5
Redundancy	47	38.5	37.6
CC(1/2)	1.000	1.000	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	277	25 % PEG-6000, 100 mM MES-Na, pH 6.5